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Cytocompatibility assessment and functional analysis of cellular responses. (A) Schematic workflow of the CCK-8 cell viability assay protocol. (B) Column chart comparing CCK-8 absorbance values (450 nm) across experimental groups. (C) Confocal microscopy images of LIVE/DEAD-stained cells. Green: Calcein-AM labeled viable cells. Red: Propidium iodide (PI) positive dead cells. Scale bar, 200 μm. (D) Fluorescent phalloidin/DAPI staining of cytoskeletal organization. Scale bar, 100 μm. (E) Dihydroethidium (DHE) probe-labeled intracellular ROS visualization under CLSM. Scale bar, 100 μm. (F) Quantitative analysis of ROS-positive cell percentage. (G) Flow cytometry histograms of DHE fluorescence intensity. (H) Hypoxia detection via [Ru(dpp) 3 ] <t>Cl</t> <t>2</t> probe under CLSM. Scale bar, 100 μm. (I) Hypoxic cell population statistics across treatment groups. (J) Flow cytometric quantification of [Ru(dpp) 3 ]Cl 2 fluorescence. All data are presented as mean ± SD, n ≥ 3. Groups that do not share the same letter are significantly (P < 0.05) different from each other.
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Cytocompatibility assessment and functional analysis of cellular responses. (A) Schematic workflow of the CCK-8 cell viability assay protocol. (B) Column chart comparing CCK-8 absorbance values (450 nm) across experimental groups. (C) Confocal microscopy images of LIVE/DEAD-stained cells. Green: Calcein-AM labeled viable cells. Red: Propidium iodide (PI) positive dead cells. Scale bar, 200 μm. (D) Fluorescent phalloidin/DAPI staining of cytoskeletal organization. Scale bar, 100 μm. (E) Dihydroethidium (DHE) probe-labeled intracellular ROS visualization under CLSM. Scale bar, 100 μm. (F) Quantitative analysis of ROS-positive cell percentage. (G) Flow cytometry histograms of DHE fluorescence intensity. (H) Hypoxia detection via [Ru(dpp) 3 ] <t>Cl</t> <t>2</t> probe under CLSM. Scale bar, 100 μm. (I) Hypoxic cell population statistics across treatment groups. (J) Flow cytometric quantification of [Ru(dpp) 3 ]Cl 2 fluorescence. All data are presented as mean ± SD, n ≥ 3. Groups that do not share the same letter are significantly (P < 0.05) different from each other.
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Cytocompatibility assessment and functional analysis of cellular responses. (A) Schematic workflow of the CCK-8 cell viability assay protocol. (B) Column chart comparing CCK-8 absorbance values (450 nm) across experimental groups. (C) Confocal microscopy images of LIVE/DEAD-stained cells. Green: Calcein-AM labeled viable cells. Red: Propidium iodide (PI) positive dead cells. Scale bar, 200 μm. (D) Fluorescent phalloidin/DAPI staining of cytoskeletal organization. Scale bar, 100 μm. (E) Dihydroethidium (DHE) probe-labeled intracellular ROS visualization under CLSM. Scale bar, 100 μm. (F) Quantitative analysis of ROS-positive cell percentage. (G) Flow cytometry histograms of DHE fluorescence intensity. (H) Hypoxia detection via [Ru(dpp) 3 ] Cl 2 probe under CLSM. Scale bar, 100 μm. (I) Hypoxic cell population statistics across treatment groups. (J) Flow cytometric quantification of [Ru(dpp) 3 ]Cl 2 fluorescence. All data are presented as mean ± SD, n ≥ 3. Groups that do not share the same letter are significantly (P < 0.05) different from each other.

Journal: Materials Today Bio

Article Title: Multimodal antibacterial microneedles with nanozymes mediated ROS-scavenging and oxygen-generation synergistic photothermal/photodynamic therapy to promote diabetic ulcer healing

doi: 10.1016/j.mtbio.2025.102368

Figure Lengend Snippet: Cytocompatibility assessment and functional analysis of cellular responses. (A) Schematic workflow of the CCK-8 cell viability assay protocol. (B) Column chart comparing CCK-8 absorbance values (450 nm) across experimental groups. (C) Confocal microscopy images of LIVE/DEAD-stained cells. Green: Calcein-AM labeled viable cells. Red: Propidium iodide (PI) positive dead cells. Scale bar, 200 μm. (D) Fluorescent phalloidin/DAPI staining of cytoskeletal organization. Scale bar, 100 μm. (E) Dihydroethidium (DHE) probe-labeled intracellular ROS visualization under CLSM. Scale bar, 100 μm. (F) Quantitative analysis of ROS-positive cell percentage. (G) Flow cytometry histograms of DHE fluorescence intensity. (H) Hypoxia detection via [Ru(dpp) 3 ] Cl 2 probe under CLSM. Scale bar, 100 μm. (I) Hypoxic cell population statistics across treatment groups. (J) Flow cytometric quantification of [Ru(dpp) 3 ]Cl 2 fluorescence. All data are presented as mean ± SD, n ≥ 3. Groups that do not share the same letter are significantly (P < 0.05) different from each other.

Article Snippet: [Ru(dpp) 3 ] Cl 2 was purchased from MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Functional Assay, CCK-8 Assay, Viability Assay, Confocal Microscopy, Staining, Labeling, Flow Cytometry, Fluorescence